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Figure 8 | BMC Microbiology

Figure 8

From: Sensitive multiplex PCR assay to differentiate Lyme spirochetes and emerging pathogens Anaplasma phagocytophilum and Babesia microti

Figure 8

Multiplex assay using 1.5 ml human blood spiked with serial dilutions of Lyme spirochetes can recover and quantitate B. burgdorferi . (A) B. burgdorferi were detected consistently in all replicates when ≥5 bacteria were present per ~75 μl of blood, i.e., when 5 μl of total 100 μl DNA recovered from 1.5 ml spiked blood was isolated without additional manipulation. Detection of human Actin A1 was not affected in the multiplex assay, as expected (data not shown). (B) Improvement in recovery and quantitation of B. burgdorferi after 48 h culture of Lyme spirochetes spiked human blood in BSKII + RS medium at 33°C was not significant.

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