Figure 6
Sensitivity of detection of tick-borne pathogens B. burgdorferi, B. microti , and A. phagocytophilum are not affected in the presence of excess of other pathogens. (A) One thousand copies of B. microti and A. phagocytophilum genomic DNA were accurately detected in the triplex assay despite 103-fold excess of copy number of B. burgdorferi genomic DNA. (B) Detection of ten B. burgdorferi recA amplicon copies was not affected in the triplex assay even in the presence of 100-fold excess of copy number of both B. microti and A. phagocytophilum genomic DNA.