Table 2 The purification table of C-His-Rv2135c from 1 liter culture of E. coli BL21(DE3)-35c
Step | Protein (mg) | Activity (U) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
Clarified extract | 464 | 18.60 | 0.04 | 1.00 | 100 |
Eluted fractions from IMAC | 50.40 | 11.60 | 0.23 | 5.60 | 62.40 |