Figure 3

12.5% SDS-PAGE of C-His-Rv0489 expressed in E. coli BL21(DE3) with or without induction and its purified form. Lane 1: 9 μl of protein ladder (Fermentas SM0431). Lane 2: 15 μg of crude lysate of E. coli BL21(DE3) without any plasmid. Lane 3: 20 μg of crude lysate of E. coli BL21(DE3)-89 before induction with IPTG. Lane 4: 20 μg of crude lysate of BL21(DE3)-89 after induction with 0.03 mM IPTG overnight at 18°C. The arrow indicates the expressed recombinant protein, C-His-Rv0489. Lane 5: 3.5 μg of recombinant C-His-Rv0489 eluted from IMAC column.