Table 2 Oligonucleotide primers
Oligonucleotide primers | Sequence (designed restriction sites are underlined) |
|---|---|
PhaP1 NdeI | 5′-GGAATTCCATATGATGTTCAAGGTTGAAGACTT-3′ |
PhaP1 XhoI | 5′-CCGCTCGAGTTAGTTGGCGGCCGGGGTGA-3′ |
PhaP2 NdeI | 5′-GGAATTCCATATGGTGAGTGATGCCGATATGAA-3′ |
PhaP2 XhoI | 5′-CCGCTCGAGTCAAGCAGCCCTATGCAGAA-3′ |
PhaP3 NdeI | 5′-GGAATTCCATATGATGATCGAACCGAAAATCGA-3′ |
PhaP3 XhoI | 5′-CCGCTCGAGTCAGGATTTGCCCTTGCCCG-3′ |
PhaP4 NdeI | 5′-GGAATTCCATATGATGACAGGTGCGACTGATCC-3′ |
PhaP4 HindIII | 5′-CCCAAGCTTTCAGGCGGCGGGCGGGTAGA-3′ |
PhaR NdeI | 5′-GGAATTCCATATGGCGAAATCAGACCAACCCAC-3′ |
PhaR HindIII | 5′-CCCAAGCTTCTACTCTTCCTTCTTCGACA-3′ |
RTphbA1-F | 5′-CATCGCCGTCAACAAGGA-3′ |
RTphbA1-R | 5′-CCGCTTCTGCATCTCGAAC-3′ |
RTphbA2-F | 5′-AAGAAGGCCGGCTGGAA-3′ |
RTphbA2-R | 5′-CCATTGACGTTGACCTTGGA-3′ |
RTphbB1-F | 5′-TCGAACTACGACGCCTGTG-3′ |
RTphbB1-R | 5′-ATGCCGTCCTTGGTGATG-3′ |
RTphbB2-F | 5′-CCGAAGGCGTGAAGAAGGT-3′ |
RTphbB2-R | 5′-GAACAGCGAGCCGAGATTG-3′ |
RTphbC1-F | 5′-GCTCTGGGAAAACATCTGGAAC-3′ |
RTphbC1-R | 5′-TTGGTGATGGTGCGGAAA-3′ |
RTphbC2-F | 5′-GGACGACTACGTTGAGGATGG-3′ |
RTphbC2-R | 5′-AATGGCGAGTGCGGATG-3′ |
RTphbC3-F | 5′-ATGACCGCGTCGAACCA-3′ |
RTphbC3-R | 5′-GGCACCTTGACCTTGGAGA-3′ |
RTphbC4-F | 5′-GGCGAAGACAGGCAAACA-3′ |
RTphbC4-R | 5′-CTCCATCCATCCGAACCA-3′ |
RTphbC5-F | 5′-CCGCAAAATTCCCTGGTC-3′ |
RTphbC5-R | 5′-CATCCCTGTCCTTCGCATC-3′ |
RTphaZ1-F | 5′-CCGAAGCAACGCACACA-3′ |
RTphaZ1-R | 5′-ATCCTCGGCACGATTTCC-3′ |
RTphaZ2-F | 5′-GGCACATCAAGCAGCACA-3′ |
RTphaZ2-R | 5′-AGATCCATCACCGCGAAA-3′ |
RTphaP1-F | 5′-ACGGCGACTACACCAAGAAG-3′ |
RTphaP1-R | 5′-GAAGGTCTCGTAGGCGGAAC-3′ |
RTphaP2-F | 5′-TCGCTTTTACCGAGCAGAAC-3′ |
RTphaP2-R | 5′-GTGAACTGGCTACGCAGGA-3′ |
RTphaP3-F | 5′-ATTACGGCACCAAGGTCATC-3′ |
RTphaP3-R | 5′-GTGGAGAGGTTCACGAGGTC-3′ |
RTphaP4-F | 5′-GTGCGACTGATCCATTCTCC-3′ |
RTphaP4-R | 5′-GTCCTTGAACTTGGCGTAGC-3′ |
RTphaR-F | 5′-CTTCGAGCAGGAGAACAAGG-3′ |
RTphaR-R | 5′-GATATTTCGGCACCACCATC-3′ |
RTsigA-F | 5′-CAGGCGAAGGACAAGGAAAA-3′ |
RTsigA-R | 5′-CGTCGGACAGATCGAGCAA-3′ |