Figure 1From: Evaluation of infectious titer in a candidate HSV type 2 vaccine by a quantitative molecular approachThe accumulation of HSV529 RNA after post-infection. The infected cells were lysed after each time point (3, 6, 12, 16, and 24Â hours post-infection), RNA was purified followed by DNase treatment, and RT-qPCR performed using specific primers for HSV-2. A. The accumulation of HSV529 targeting gD2 gene is shown. A linear relationship between the logarithm of the HSV529 concentration and the C T values were observed at 12 to 16Â h post-infection. B. The accumulation of HSV529 targeting ICP-27 gene is shown No linear relationship between the logarithm of the HSV529 concentration and the C T values were observed at any time points (3, 6, 12, 16, and 24Â h). As a representative, the accumulation of HSV529 RNA targeting ICP-27 after 6Â h and 12Â h post-infection is shown. C. The accumulation of HSV529 RNA targeting TK gene after 3Â h and 6Â h post-infection is shown. No linear relationship between the logarithm of the HSV529 concentration and the C T values were observed at any time points (3, 6, 12, 16, and 24Â h). dil:dilution.Back to article page