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Table 2 Strains and plasmids/cosmids used in this work

From: Identification of new developmentally regulated genes involved in Streptomyces coelicolorsporulation

Strains/plasmids

Description

Reference

E. coli

  

DY380

∆(mrr–hsdRMSmcrBC) mcrA recA1 λ cl857, ∆(cro–bioA)<>tet

[46]

ET12567/pUZ8002

dam-13::Tn9 dcm-6 hsdM; carries RK2 derivative with defective oriT for plasmid mobilization, Kanr

[45]

GM2929

dam-13::Tn9 dcm-6 hsdR2 recF143

M. Marinus, Univ. of Massachussetts Medical School

S. coelicolor A3(2)

  

M145

Prototrophic, SCP1- SCP2- Pgl+

[45]

J2401

M145 whiA::hyg

[15]

J2408

M145 ∆whiH::ermE

[15]

K300

M145 ∆SCO1774-1773::vph

This work

K301

M145 ∆SCO1773::vph

This work

K302

M145 ∆SCO3857::vph

This work

K303

M145 ∆SCO4157::aac(3)IV

This work

K316

M145 ∆SCO0934::aac(3)IV

This work

K317

M145 ∆SCO7449-7451::aac(3)IV

This work

K318

M145 ∆SCO1195-1196::Ωaac

This work

K319

M145 ∆SCO4421::Ωaac

This work

Plasmids/cosmids

  

pCR-BluntII

Cloning vector

Invitrogen

pIJ773

Source of apramycin resistance cassette, aac(3)IV, oriT

[47]

pIJ780

Source of viomycin resistance cassette, vph, oriT

[47]

pHP450Ωaac

Source of apramycin resistance cassette, Ωaac

[48]

pIJ2925

pUC-derived E. coli vector with a modified polylinker; bla

[49]

pOJ260

Mobilizable vector, no replication or integration in S. coelicolor, Aprar

[50]

pSET152

Mobilizable vector, integrates at ϕC31 attB site, Aprar

[50]

pIJ82

Derivative of pSET152, Hygr

Helen Kieser, JIC, Norwich, UK

pRT801

Mobilizable vector, integrates at ϕBT1 attB site, Aprar

[51]

pIJ6902

Expression vector, thiostrepton-inducible tipAp promoter, integrates at ϕC31 attB site, Aprar

[52]

pKF218

pRT801 containing SCO1775-1773 with part of upstream region

This work

pKF219

pOJ260 containing SCO1775-1773 with part of upstream region

This work

pKF278

pIJ82 containing SCO7449-7451 with part of upstream region

This work

pKF210

Vector for cloning promoters upstream reporter gene encoding mCherry, based on pIJ6902

This work

pKF212

Promoter region of SCO0934 translationally fused to mCherry

This work

pKF213

Promoter region of SCO1773 translationally fused to mCherry

This work

pKF214

Promoter region of SCO1774 translationally fused to mCherry

This work

pKF215

Promoter region of SCO3857 translationally fused to mCherry

This work

pKF216

Promoter region of SCO4157 translationally fused to mCherry

This work

pKF217

Promoter region of SCO4421 translationally fused to mCherry

This work

M10

Cosmid containing SCO0934a

[53]

I51

Cosmid containing SCO1773 and SCO1774a

[53]

H69

Cosmid containing SCO3857a

[53]

D84

Cosmid containing SCO4157a

[53]

6 F11

Cosmid containing SCO4421a

[53]

5C11

Cosmid containing SCO7449-7451a

[53]

G11A

Cosmid containing SCO1195-1196a

[53]

  1. aCosmid used to delete corresponding gene and to amplify promoter regions and regions used for complementation.