From: Identification of new developmentally regulated genes involved in Streptomyces coelicolorsporulation
Strains/plasmids | Description | Reference |
---|---|---|
E. coli | ||
DY380 | ∆(mrr–hsdRMS–mcrBC) mcrA recA1 λ cl857, ∆(cro–bioA)<>tet | [46] |
ET12567/pUZ8002 | dam-13::Tn9 dcm-6 hsdM; carries RK2 derivative with defective oriT for plasmid mobilization, Kanr | [45] |
GM2929 | dam-13::Tn9 dcm-6 hsdR2 recF143 | M. Marinus, Univ. of Massachussetts Medical School |
S. coelicolor A3(2) | ||
M145 | Prototrophic, SCP1- SCP2- Pgl+ | [45] |
J2401 | M145 whiA::hyg | [15] |
J2408 | M145 ∆whiH::ermE | [15] |
K300 | M145 ∆SCO1774-1773::vph | This work |
K301 | M145 ∆SCO1773::vph | This work |
K302 | M145 ∆SCO3857::vph | This work |
K303 | M145 ∆SCO4157::aac(3)IV | This work |
K316 | M145 ∆SCO0934::aac(3)IV | This work |
K317 | M145 ∆SCO7449-7451::aac(3)IV | This work |
K318 | M145 ∆SCO1195-1196::Ωaac | This work |
K319 | M145 ∆SCO4421::Ωaac | This work |
Plasmids/cosmids | ||
pCR-BluntII | Cloning vector | Invitrogen |
pIJ773 | Source of apramycin resistance cassette, aac(3)IV, oriT | [47] |
pIJ780 | Source of viomycin resistance cassette, vph, oriT | [47] |
pHP450Ωaac | Source of apramycin resistance cassette, Ωaac | [48] |
pIJ2925 | pUC-derived E. coli vector with a modified polylinker; bla | [49] |
pOJ260 | Mobilizable vector, no replication or integration in S. coelicolor, Aprar | [50] |
pSET152 | Mobilizable vector, integrates at ϕC31 attB site, Aprar | [50] |
pIJ82 | Derivative of pSET152, Hygr | Helen Kieser, JIC, Norwich, UK |
pRT801 | Mobilizable vector, integrates at ϕBT1 attB site, Aprar | [51] |
pIJ6902 | Expression vector, thiostrepton-inducible tipAp promoter, integrates at ϕC31 attB site, Aprar | [52] |
pKF218 | pRT801 containing SCO1775-1773 with part of upstream region | This work |
pKF219 | pOJ260 containing SCO1775-1773 with part of upstream region | This work |
pKF278 | pIJ82 containing SCO7449-7451 with part of upstream region | This work |
pKF210 | Vector for cloning promoters upstream reporter gene encoding mCherry, based on pIJ6902 | This work |
pKF212 | Promoter region of SCO0934 translationally fused to mCherry | This work |
pKF213 | Promoter region of SCO1773 translationally fused to mCherry | This work |
pKF214 | Promoter region of SCO1774 translationally fused to mCherry | This work |
pKF215 | Promoter region of SCO3857 translationally fused to mCherry | This work |
pKF216 | Promoter region of SCO4157 translationally fused to mCherry | This work |
pKF217 | Promoter region of SCO4421 translationally fused to mCherry | This work |
M10 | Cosmid containing SCO0934a | [53] |
I51 | Cosmid containing SCO1773 and SCO1774a | [53] |
H69 | Cosmid containing SCO3857a | [53] |
D84 | Cosmid containing SCO4157a | [53] |
6 F11 | Cosmid containing SCO4421a | [53] |
5C11 | Cosmid containing SCO7449-7451a | [53] |
G11A | Cosmid containing SCO1195-1196a | [53] |