Biochemical and molecular characterization of Treponema phagedenis-like spirochetes isolated from a bovine digital dermatitis lesion

Table 4 Comparison of Isolate 4A to other treponemes using Genome-To-Genome Distance Calculator ( http://ggdc.gbdp.org/ )

Reference Sequence†	Comparison Program	DDH% estimate**
Treponema phagedenis F0421* 2.83 Mb, AEFH00000000.1	BLAT	82.11
Treponema phagedenis F0421* 2.83 Mb, AEFH00000000.1	NCBI-BLAST	84.59
Treponema succinifaciens DSM 2489	"	52.5
Complete chromosome, 2.73 Mb, NC_015385.1	"	52.5
Treponema azotonutricium ZAS 9	"	47.15
Complete chromosome, 3.85 Mb, NC_015577.1	"	47.15
Treponema primitia ZAS 2	"	45.7
Complete chromosome, 4.05 Mb, NC_015578.1	"	45.7
Treponema brennaborense DSM 12	"	35.64
Complete chromosome, 3.05 Mb, NC_015500.1	"	35.64
Treponema denticola ATCC 35405	"	29.34
Complete chromosome, 2.84 Mb, NC_002967.9	"	29.34
Treponema paraluiscuniculi Cuniculi A	"	25.82
Complete chromosome, 1.13 Mb, NC_015714.1	"	25.82
Treponema pallidum subsp. pallidum SS14	"	25.75
Complete chromosome, 1.14 Mb, NC_010741.1	"	25.75

†All comparisons used 60 Contigs assembled for Isolate 4A as Query and report results using Formula 2 (Identities/HSP length).
**Regression based. DNA-DNA Hybridization (DDH%) estimates ≤70% indicate organisms compared represent different species. Estimates >70% indicate organisms represent same species.
*277 Contigs for Treponema phagedenis F0412 were used as reference sequence.

ISSN: 1471-2180