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Table 2 Evaluation of purification procedures and their modifications by fluorescence microscopy

From: Development of a flow-fluorescence in situhybridization protocol for the analysis of microbial communities in anaerobic fermentation liquor

Procedure

Cell aggregates present

Maximum cell aggregate size1)

Abiotic particles present

Abiotic particles covered with cells

1-C1-S1-H1-F1

yes

+++

yes

no

1-C1-S1-H2-F1

yes

++

yes

no

1-C2-S1-H1-F1

yes

++

yes

no

1-C2-S1-H2-F1

yes

+

yes

no

1-C2-S2-H1-F1

no

-

yes

no

1-C2-S2-H1-F2

no

-

no

no

2-C1-S1-H1

yes

+++

yes

yes

2-C1-S1-H2

yes

+++

yes

yes

3-C1-S1-H1

yes

+++

yes

yes

3-C1-S1-H2

yes

++

yes

yes

3-C1-S2-H1

yes

++

yes

yes

3-C1-S2-H2

yes

+

yes

yes

3-C2-S1-H1

yes

+++

yes

yes

3-C2-S1-H2

yes

++

yes

yes

3-C2-S2-H1

yes

++

yes

yes

3-C2-S2-H2

yes

++

yes

yes

3-C3-S1-H1

yes

++

yes

yes

3C3-S1-H2

yes

++

yes

yes

3-C3-S2-H1

yes

++

yes

yes

3-C3-S2-H2

yes

+

yes

yes

4-C1-H1

yes

+++

yes

yes

5-C1-S1-H1

yes

+++

yes

yes

5-C1-S2-H1

yes

+++

yes

yes

5-C1-S1-H2

yes

++

yes

yes

5-C1-S2-H2

yes

++

yes

yes

5-C2-S1-H1

yes

+++

yes

yes

5-C2-S2-H1

yes

+++

yes

yes

5-C2-S1-H2

yes

++

yes

yes

5-C2-S2-H2

yes

+

yes

yes

6-C1-S1-H1

yes

++

yes

yes

  1. 1) +++ = ≥ 52 μm2; ++ = ≥ 24 μm2; + = ≥ 6 μm2; - = no cell aggregates. The size of cell aggregates was determined by microscopic field analyses using an ocular micrometer at 630× magnification. One field covered an area of 5.76 μm2.
  2. Denomination of procedures is according to Table 1. The optimal combination is given in italics.