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Table 1 Purification procedures and modifications

From: Development of a flow-fluorescence in situhybridization protocol for the analysis of microbial communities in anaerobic fermentation liquor

Procedures

References

Detergents

Detergent concentrations (C)

Ultrasound treatment (S)1)

Homogenization (H)2)

Filtration (F)

1

S.B. Singh-Verma (1968), LR. Bakken (1985)

Sodium hexametaphosphate

C1) 0,2% (w/v)

S1) 40 W, 60 sec, 5 impulses/sec (different repetitions)

H1) none

F1 none

  

C2) 0,5% (w/v)

S2) 65 W, 60 sec, 5 impulses/sec (different repetitions)

H2) 60 sec, speed 5 (different repetitions)

F2) 12–15 μm filter

2

S.B. Singh-Verma (1968), LR. Bakken (1985)

Bromhexine hydrochloride

C1) 0,2% (w/v)

S1) 40 W, 60 sec + 65 W, 60 sec, 5 impulses/sec

H1) none

n.a.

    

H2) 2× 60 sec, speed 5

 

3

W.B. Yoon and R.A. Rosson (1990)

Tween

C1) 5 μg/ml

S1) 15 W, 30 sec, 5 impulses/sec

H1) none

n.a.

  

C2) 10 μg/ml

S2) 35 W, 30 sec, 5 impulses/sec

H2) 5 min, speed 5

 
  

C3) 25 μg/ml

   

4

E.L Schmidt (1974)

Tween 80 + 0.007 g ml-1 flocculation reagent (Ca (OH)2: MgCO3 (2:5))

C1) 25 μl/ml

n.a.

n.a.

n.a.

5

O. Resina-Pelfort et al. (2003)

Triton X-100

C1) 10 μg/ml

S1) 35 W, 30 sec, 5 impulses/sec

H1) none

n.a.

  

C2) 20 μg/ml

S2) 45 W, 30 sec, 5 impulses/sec

H2) 5 min, speed 5

 

6

L R. Bakken (1985)

Sodium pyrophosphate

C1) 0,2% (w/v)

S1 3× 40 W, 60 sec, 5 impulses/sec

H1) 3× 60 sec, speed 5

n.a.

  1. n.a. = not applied.
  2. 1)using the Sonoplus GW2070 (Bandelin, Germany).
  3. 2)using the dispersion unit VDI12 for 0.1 - 5.0 ml volumes (VWR, Germany).
  4. C1-3, H1-2, S1-2 and F1-2 indicate variations of the original protocols tested for their eligibility on samples from pure cultures and the UASS biogas reactor.