Strain or plasmid | Description | Reference |
---|---|---|
V. anguillarum strains | Â | Â |
M93sm | Spontaneous Smr mutant of M93 (serotype O2a); parental strain isolated from a diseased ayu (Plecoglossus altivelis) from Lake Biwa, Japan | [2] |
JR1 | Smr Cmrvah1; insertional vah1 mutant of M93Sm | [8] |
XM21 | Smr Cmr Tcrvah1+; vah1 complement strain of JR1 | This study |
S262 | Smr Cmrplp; insertional plp mutant of M93Sm | This study |
XM31 | Smr Cmr Tcrplp+; plp complement strain of S262 | This study |
S123 | Smr CmrrtxA; insertional rtxA mutant of M93Sm | [9] |
JR3 | Smr Cmr Kmrvah1 plp; insertional vah1mutant of JL01 | [8] |
S183 | Smr Cmr Kmrvah1 rtxA; insertional rtxA mutant of S171 | [9] |
XM62 | Smr Cmr Kmr Tcrvah1+ rtxA; vah1 complement strain of S183 | This study |
S187 | Smr Cmr Kmrplp rtxA; insertional rtxA mutant of JL01 | This study |
XM90 | Smr Cmr Kmrvah1 plp rtxA; insertional plp mutant of S264 | This study |
XM93 | Smr Cmr Kmr Tcrvah1 plp + rtxA; plp complement strain of XM90 | This study |
JL01 | Smr Kmrplp; mini-Tn10Km insertion into plp | [8] |
S171 | Smr Kmrvah1; allelic exchange vah1 mutant | [9] |
S264 | Smr Kmrvah1 rtxA; allelic exchange vah1 and rtxA mutant | This study |
E. coli strains | Â | Â |
Sm10 | thi thr leu tonA lacY supE recA RP4-2-Tc::Mu::Km (λ pir) | [21] |
S253 | Kmr Cmr; Sm10 containing plasmid pNQ705-plp | This study |
S118 | Kmr Cmr; Sm10 containing plasmid pNQ705-rtxA | [9] |
S250 | Kmr Cmr; Sm10 containing plasmid pDM4-rtxA5' | This study |
S252 | Kmr Cmr; Sm10 containing plasmid pDM4-rtxA5'-rtxA3' | This study |
U21 | Kmr Apr Tcr; Sm10 containing plasmid pSUP202-vah1 | This study |
U31 | Kmr Apr Tcr; Sm10 containing plasmid pSUP202-plp | This study |
M15 | NalS StrS RifSthi–lac–ara+gal+mtl– F–recA+uvr+lon+ (pREP4) | QIAGEN, USA |
S238 | Kmr Apr; M15 containing plasmid pQE30UA-plp | This study |
S269 | Kmr Apr; M15 containing plasmid pQE60-plp | This study |
Plasmid | Â | Â |
PCR2.1 | Kmr Apr; Cloning vector | Invitrogen, USA |
pNQ705-1 | Cmr; suicide vector with R6K origin | [22] |
pNQ705-vah1 | Cmr; for insertional vah1mutation | [8] |
pNQ705-plp | Cmr; for insertional plp mutation | This study |
pNQ705-rtxA | Cmr; for insertional rtxA mutation | [9] |
pDM4 | Cmr SacBCr; suicide vector with R6K origin | [11] |
pDM4-rtxA5'-rtxA3' | Cmr SacBCr; for allelic exchange rtxA mutation | This study |
pSUP202 | Cmr Apr Tcr; E. coli – V. anguillarum shuttle vector | [21] |
pSUP202-vah1 | Apr Tcr; for complementation of vah1 | This study |
pSUP202-plp | Apr Tcr; for complementation of plp | This study |
pQE-30 UA | Apr; expression vector with N-terminal His6-tag | QIAGEN, USA |
pQE30UA-plp | Apr; for expression of rPlp that is used to make anti-Plp | This study |
pQE60 | Apr; expression vector with C-terminal His6-tag | QIAGEN, USA |
pQE-60-plp | Apr; for expression of rPlp for enzymatic activity analysis | This study |