Figure 1From: Using phage display selected antibodies to dissect microbiomes for complete de novo genome sequencing of low abundance microbesA phage display derived single chain fragment (scFv) was selected that binds Lactobacillus acidophilus (L.a.) specifically. Various bacterial species (see Table 1 for abbreviations) were mixed with the α-La scFv-SV5-GFP-s11 fusion protein and stained with α-SV5-IgG-PE and/or GFP1-10. Binding specificity was confirmed using both standard (A) and imaging (B) flow cytometry (BF = Bright Field, GFP = Green Fluorescent Protein, PE = Phycoerytherin).Back to article page