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Table 1 Strains and plasmids used in this study

From: Characterization of the Pseudomonas aeruginosametalloendopeptidase, Mep72, a member of the Vfr regulon

Strains or plasmids

Description

Source (Reference)

Pseudomonas aeruginosa

PAO1

Prototrophic strain

[31]

PW5661

PA2783-E12::ISlacZ/hah; insertion at bp 27 of 1800 in the ORF; Tcr

[28]

PAOΔvfr (PAO9001)

vfr deletion of PAO1; Gmr

[32]

PAO-R1

ΔlasR::tet derivative of PAO1; Tcr

[33]

Escherichia coli

DH5α

Φ80lacZΔM15 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1

Invitrogen

CC118

F− Δ(ara-leu)7697 araD139 ΔlacX74 galE galK ΔphoA20 thi rpsE rpoB argE(Am) recA1 appR1

[34]

CC102

F42 lacI3 zzf-2::TnphoA/CC118

[34]

LMG194

F− ΔlacX74 galE galK thi rpsL ΔphoA (PvuII) Δara714 leu::Tn10

Invitrogen

Plasmids

pUCP19

E. coli-P. aeruginosa shuttle vector; Cbr

[35]

pKF917

pUCP19 carrying vfr; Cbr

[15]

pCR™2.1-TOPO®

3.9 kbp TA cloning vector; Cbr, Kmr

Invitrogen

pAB1

pCR2.1-TOPO carrying PA2783; Cbr , Kmr

This study

pAB2

pUCP19 carrying PA2783 expressed from P lac ; Cbr

This study

pAB3

pAB2 carrying a phoA fusion; Cbr, Kmr

This study

pBAD/HisC

pBR322-derived expression vector in which cloned genes are expressed from the araBAD promoter (PBAD); Cbr

Invitrogen

pAB4

pBAD/HisC carrying PA2783 expressed from PBAD; Cbr

This study

  1. ORF, open-reading frame; r, resistant; Cb, carbenicillin; Gm, gentamicin; Km, kanamycin; Tc, tetracycline.