Strains or plasmids | Description | Source (Reference) |
---|---|---|
Pseudomonas aeruginosa | ||
PAO1 | Prototrophic strain | [31] |
PW5661 | PA2783-E12::ISlacZ/hah; insertion at bp 27 of 1800 in the ORF; Tcr | [28] |
PAOΔvfr (PAO9001) | vfr deletion of PAO1; Gmr | [32] |
PAO-R1 | ΔlasR::tet derivative of PAO1; Tcr | [33] |
Escherichia coli | ||
DH5α | Φ80lacZΔM15 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Invitrogen |
CC118 | F− Δ(ara-leu)7697 araD139 ΔlacX74 galE galK ΔphoA20 thi rpsE rpoB argE(Am) recA1 appR1 | [34] |
CC102 | F42 lacI3 zzf-2::TnphoA/CC118 | [34] |
LMG194 | F− ΔlacX74 galE galK thi rpsL ΔphoA (PvuII) Δara714 leu::Tn10 | Invitrogen |
Plasmids | ||
pUCP19 | E. coli-P. aeruginosa shuttle vector; Cbr | [35] |
pKF917 | pUCP19 carrying vfr; Cbr | [15] |
pCR™2.1-TOPO® | 3.9 kbp TA cloning vector; Cbr, Kmr | Invitrogen |
pAB1 | pCR2.1-TOPO carrying PA2783; Cbr , Kmr | This study |
pAB2 | pUCP19 carrying PA2783 expressed from P lac ; Cbr | This study |
pAB3 | pAB2 carrying a phoA fusion; Cbr, Kmr | This study |
pBAD/HisC | pBR322-derived expression vector in which cloned genes are expressed from the araBAD promoter (PBAD); Cbr | Invitrogen |
pAB4 | pBAD/HisC carrying PA2783 expressed from PBAD; Cbr | This study |