Figure 4From: Characterization of the Pseudomonas aeruginosametalloendopeptidase, Mep72, a member of the Vfr regulonPA2783 is exported to the outer membrane in PAO1. Overnight cultures of PAO1 were subcultured in LB broth and grown to the time points indicated on the graph. Cells from 1-ml samples were fractionated and the level of alkaline phosphatase activity in the outer membrane fraction was determined as previously described. Values represent the means of three independent experiments ± SEM. ***P <0.001.Back to article page