Skip to main content

Table 2 CcsX and DsbD homologs identified in four anammox genera

From: Identification of the type II cytochrome cmaturation pathway in anammox bacteria by comparative genomics

Homolog

Anammox genus

Gene product

Length (aa)

BLAST*

HHPRED**

HMMER**

Motif

Additional Cys residues

TMHs

Signal peptide

CcsX

Kuenenia

kuste0860

161

✓

✓

✓

CX2C

1

✓

✗

kuste0967

166

✓

✓

✓

CX2C

1

✓

✗

kuste3827

164

✓

✓

✓

CX2C

3

✓

✓

Scalindua

scal02124

172

✓

✓

✓

CX2C

0

✓

✓

scal00014c

173

✓

✓

✓

CX2C

3

✓

✓

scal02421c

255

✓

✓

✓

CX2C

1

✓

✓

scal02845

125

✓

✓

✓

CX2C

0

✗

✗

scal00012c

185

✓

✗

✓

CX2C

1

✗

✓

scal04176

164

✓

✓

✓

CX4C

1

✗

✗

KSU-1

GAB64172.1

312

✓

✓

✓

CX2C

1

✓

✗

GAB61322.1

165

✓

✓

✓

CX2C

2

✓

✓

GAB62714.1

162

✓

✓

✓

CX2C

1

✓

✓

GAB64222.1

163

✓

✓

✓

CX2C

1

✓

✓

GAB64221.1

163

✓

✓

✓

CX2C

0

✓

✓

GAB62039.1

669

✓

✓

✓

CX2C

8

✓

✗

Brocadia

BFUL_03119

163

✓

✓

✓

CX2C

0

✓

✓

BFUL_00886

173

✓

✓

✓

CX2C

2

✗

✓

DsbD

Kuenenia

kuste2732

601

✓

✓

✓

NA

7

8

NA

kustc0946

608

✓

✓

✓

NA

8

9

NA

KSU-1

GAB61320.1

610

✓

✓

✓

NA

5

11

NA

Brocadia

BFUL_00929

610

✓

✓

✓

NA

5

9

NA

CcdA

Scalindua

scal01537

234

✓

✓

✓

NA

2

6

NA

  1. Legend: Initial blastP search of whole anammox genomes against a reference database, comprising UNIPROT entries for CcsX and DsbD was performed. Retrieved results were further analyzed with HHpred and HMMER (Additional file 6), transmembrane helices were predicted with TMHMM, potential signal peptides were annotated using SignalP 4.1, and conserved motifs together with critical residues were identified manually. TMHs: transmembrane helices; (*): E-value cut off set at 10-6; (**): E-value cut off set at 10-3; (✓): significant annotation and/or identification; (✗): absence of significant hits and/or transmembrane helix and/or signal peptides; (NA): not applicable.