Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid

Table 3 Description of the pX1 :: CMY transconjugants obtained from the YU39 donor

Recipient	pX1 ::CMY colony	pX1 PCR typing						CMY region^a	Insertion region^b	Second round conjugation^c
		*oriX1*	ydgA	taxB	taxC	ddp3	046-047			Original	DH5α
HB101	IC2	+	+	+	+	+	-	Large	046-047	10^-1	1 to 10^-2
	IIC1	+	-	+	+	+	+	Short	ND	10^-1	10^-1 to 10^-2
	IIIC10	+	+	+	+	+	+	Short	stbE	10^-1	10^-1 to 10^-2
HB101 (pSTV::Km)	ID1	+	+	+	+	+	-	Large	046-047	10^-1	1 to 10^-1
HB101 (pSTV::Km)	IIID2	+	+	+	+	+	-	Large	046-047	10^-2 to 10^-4	10^-4 to 10^-7
	IVD8	+	+	+	+	+	+	Short	stbE	10^-1 to 10^-2	10^-1
LT2	IIE2	+	+	+	+	+	+	Short	stbE	10^-1 to 10^-5	10^-1
(pSTV::Km)	IIIE4	+	+	+	+	+	+	Large	ND	10^-4 to 10^-7	1 to 10^-1

^aThe long CMY region includes from ISEcp1 to hypothetical protein 0093, and the short region includes from ISEcp1 to sugE (see text for details).
^bSection of pX1 where the CMY region was inserted (see text for details). ND, not determined.
^cTransconjugants were challenged for a second round of conjugation using as recipients the original recipient strain (original) or DH5α. The frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.