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Table 1 Bacterial strains and plasmids used in this work

From: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid

Strain

Plasmids (kb)

Feature

Salmonella

  

YU39 (ST213)

pA/C (150), p100 (100), pX1 (40), pColE1-like (5), p3 (3)

Donor

SO1 (ST19)

pSTV::Km (94), p80 (80)

Recipient

LT2 (ST19)

pSTV::Km (94)

Recipient

E. coli

  

DH5α

 

Recipient

HB101

 

Recipient

HB101pSTV

pSTV::Km

Recipient

DH5α

pA/C

Wild-type pA/C, donor

DH5α

pA/C, pSTV::Km

Stability assays

DH5α

pX1

Wild-type pX1

Transconjugants

  

SO1

  

IA4

pA/C

Re-arranged pA/C

IA5

pA/C

Re-arranged pA/C

IA9

pA/C

Re-arranged pA/C

IIA4

pA/C + pX1

pA/C and pX1 co-integrate

HB101

  

IC2

pX1::CMY

pX1 with the transposed CMY region

IIC1

pX1::CMY

pX1 with the transposed CMY region

IIIC9

pA/C + pX1

pA/C and pX1 co-integrate

IIIC10

pX1::CMY

pX1 with the transposed CMY region

IVC8

pA/C + pX1

pA/C and pX1 co-integrate

HB101pSTV ::Km

  

ID1

pX1::CMY

pX1 with the transposed CMY region

IID2

pX1::CMY

pX1 with the transposed CMY region

IIID8

pA/C + pX1

pA/C and pX1 co-integrate

IVD2

pA/C + pX1

pA/C and pX1 co-integrate

IVD8

pX1::CMY

pX1 with the transposed CMY region

LT2

  

IIE2

pX1::CMY

pX1 with the transposed CMY region

IIIE4

pX1::CMY

pX1 with the transposed CMY region

IIIE9

pA/C + pX1

pA/C and pX1 co-integrate

DH5α

  

221-1

pA/C + pX1

pA/C and pX1 co-integrate

221-10

pA/C + pX1

pA/C and pX1 co-integrate

225-1

pA/C + pX1

pA/C and pX1 co-integrate

225-7

pA/C + pX1

pA/C and pX1 co-integrate

pX1 mutants

  

DH5α

pX1ydgA::Tn5

Tn5 transposon insertion

DH5α

pX1taxB::Km

taxB site-directed mutant

DH5α

pA/C, pX1ydgA::Tn5

Donor

DH5α

pA/C,pX1taxB::Km

Donor