Figure 1

Growth phenotype of the gpsA knockout strain. S. aureus strain PDJ28 (ΔgpsA) was grown in RN medium to an OD₆₀₀ of 0.5 and the cells were harvested and washed to remove the glycerol supplement. The culture was split and resuspended in media either with or without 0.1% glycerol, and growth was followed as a function of time. The growth curve is representative example of the data obtained in duplicate experiments. The figure inset shows the multiplex PCR genotyping of the wild-type gpsA gene (528 bp) in strain RN4220 and the inactivated gpsA allele (394 bp) in strain PDJ28 as described under Methods.