Figure 1From: Lipopolysaccharide (LPS)-induced autophagy is involved in the restriction of Escherichia coliin peritoneal mesothelial cellsCharacterization and analysis of cell viability in HMrSV5 cells. (A) Confluent PMCs were positive for cytokeratin 18 and vimentin. Scale bars: 50 μm. (B) Cell viability was determined by MTT assay. The left panel shows the viability of HMrSV5 cells exposed to different concentrations (0, 0.1, 0.5, 1.0, 2.0 and 5.0 μg/ml) of LPS for 24 hours. The right panel shows the viability of HMrSV5 cells exposed to 1 μg/ml LPS for different times (0, 3, 6, 12, 18 and 24 hours). The results are presented as a percentage of the MTT absorbance of untreated cells (100%). Data represent mean values ± SD (n ≥ 3). (C) Detection of cell viability by flow cytometric analysis. The upper panel shows dose responses for LPS-induced apoptosis over 24 hours in HMrSV5 cells. The lower panel shows apoptosis in cells treated with 1.0 μg/ml LPS for 0, 3, 6, 12, 18 and 24 hours.Back to article page