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Figure 3 | BMC Microbiology

Figure 3

From: Cloning and expression of an active aspartic proteinase from Mucor circinelloides in Pichia pastoris

Figure 3

SDS-PAGE analysis of the extracellular extract from recombinants X-33/pGAPZα +MCAP-2, X-33/pGAPZα+MCAP-3, X-33/pGAPZα+MCAP-5, X-33/pGAPZα+MCAP-SP1, M. circinelloides and P. pastoris X-33 (wild-type). 25 μg of the concentrated protein products were subjected on each lane of SDS-PAGE. Samples: Lane 1, molecular standards (kDa); lane 2, secreted expression from recombinant X-33/pGAPZα+MCAP-5; lane 3, P. pastoris X-33 (negative control); lane 4, X-33/pGAPZα+MCAP-2; lane 5, X-33/pGAPZα+MCAP-3; lane 6, X-33/pGAPZα+MCAP-SP1; and lane 7, secreted expression from M. circinelloides. The asterisk indicates the authentic MCAP. The arrows indicate the expressed forms (A and B) of MCAP protein.

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