C. albicansgrowth, transition, biofilm formation, and gene expression modulation by antimicrobial decapeptide KSL-W

Table 5 Gene expression (6 h) under hyphae inducing culture conditions (medium supplemented with 10% fetal calf serum, with culture incubation at 37ºC)

Gene	Untreated *C. albicans*	Amphotericin B		KSL-W 25 μg/ml		KSL-W 100 μg/ml
Gene	Fold change¹	Fold change¹	p-value²	Fold change¹	p-value²	Fold change¹	p-value²
SAP2	0.99	8.17	0.009	0.7	0.2	1.31	0.02
SAP4	0.96	2.58	0.03	0.73	0.04	0.72	0.04
SAP5	1.00	0.72	0.007	0.83	0.0004	0.56	0.006
SAP6	1.00	4.01	0.02	0.58	0.01	0.68	0.04
EAP1	1.00	6.36	0.001	0.44	0.008	0.73	0.003
EFG1	1.00	1.78	0.048	0.31	< 0.0001	0.47	0.01
NRG1	1.00	3.97	0.0005	0.37	0.001	0.37	0.05
HWP1	1.00	0.008	< 0.001	0.09	0.001	0.03	< 0.0001

¹Fold change was calculated by PCR product of the gene of interest/the PCR product of ACT1 (the house keeping gene), and normalized to the negative control of untreated C. albicans where the expression was considered equal to 1.
²P-values were obtained after comparison of test to negative control (untreated C. albicans).

ISSN: 1471-2180