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Table 1 MIC data for lacticin 3147, polymyxin B and polymyxin E alone and in combination

From: The two peptide lantibiotic lacticin 3147 acts synergistically with polymyxin to inhibit Gram negative bacteria

Organism

MIC (μg/ml)

 

Lacticin 3147

Polymyxin B

Polymyxin E

Lacticin 3147/

FIC

Lacticin 3147/

FIC

    

Polymyxin B

 

Polymyxin E

 

Salmonella Typhimurium UK1

924

0.0586

0.0586

924/0.015

1.25d

924/0.0073

1.125d

Salmonella Typhimurium LT2

231

0.3125

0.4688

No MIC

>4e

No MIC

>4e

Cronobacter sakazakii DPC 6440

>924

0.3125

0.3125

57.75/0.0781

0.250 (<0.312)*a

57.75/0.0195

0.062 (<0.125)*a

E. coli 0157:H-

231

0.0586

0.0781

28.875/0.0073

0.250a

28.875/0.0049

0.188a

E. coli DH5α

462

0.0781

0.0781

28.875/.0098

0.188a

28.875/0.0098

0.188a

E. coli EC101

462

0.0781

0.0781

14.4375/.0391

0.5a

28.875/0.0098

0.188a

E. faecium DO

0.9625

>375

>375

0.9625/23.4375

1c

0.9652/23.4375

1c

B. cereus 8079

3.85

187.5

375

1.925/23.4375

0.62b

3.85/375

2d

S .aureus 5247

15.4

187.5

>375

7.7/46.875

0.75b

15.4/23.4375

1c

  1. FIC figures have been calculated as a result of triplicate experiments and indicate asynergy, bfor partial synergy, cadditive effects, dindifference, and eantagonism between the combined antimicrobials. *FIC index which includes the reduction in lacticin 3147 MIC from the highest level tested to that which achieves an MIC in the presence of polymyxin.