Deletion of the HAMP domains from the histidine kinase CaNik1p of Candida albicans or treatment with fungicides activates the MAP kinase Hog1p in S. cerevisiae transformants

Table 2 Oligonucleotides used in this study

Primer	Sequence (5´-3´)	Used in mutation of:
F2Gln	CTAGCGAACATGTCGCAAGAGATACGTACACC	HisKA domain
R2Gln	GGTGTACGTATCTCTTGCGACATGTTCGCTAG	HisKA domain
TAsnF	CTTAACTTGGCTGGTGATGCTATTAAGTTTAC	HATPase_c domain
TAsnR	GTAAACTTAATAGCATCACCAGCCAAGTTAAG	HATPase_c domain
AAsnF	GATGTGGTGTTGATGAATGTGCAAATGCCTGTAATG	REC domain
AAsnR	CATTACAGGCATTTGCACATTCATCAACACCACATC	REC domain
HMPF1	AGGGAATATT *AAGCT* TATGAACCCCACTAAAAAACCACG	HAMP domains
HMPR1	GTTCGCGTTTTTGGATTTTTCTAG
HMPF2	TCCAAAAACGCGAACAGGAATACTGCGGCTAGAGAAGCTG
HMPR2	GCTCGGTACC *AAGCT* TTCAGTGGTGATGGTGATGATGTCC

Bold bases are those used to introduce the point mutations. Bold italic bases indicate the HindIII restriction sites. Underlined bases are the overlapping sequences recognized by the in-fusion enzyme.

ISSN: 1471-2180