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Figure 5 | BMC Microbiology

Figure 5

From: Deletion of the HAMP domains from the histidine kinase CaNik1p of Candida albicans or treatment with fungicides activates the MAP kinase Hog1p in S. cerevisiae transformants

Figure 5

The MAPK Hog1p was constitutively phosphorylated after expression of CaNIK1ΔHAMP in the strain ΔHa. Phosphorylation of Hog1p (upper panel, Hog1-P) in the strains YES, NIK, ΔHa and ΔHaH510 was detected after cultivation of the strains in SG-ura for 195 and 210 min. Fludioxonil was added after 180 min at a final concentration of 10 μg/ml. The presence of Hog1p (lower panel, Hog1) was confirmed in all strains. Hog1p appears at approximately 50 kDa.

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BMC Microbiology

ISSN: 1471-2180

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