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Table 1 Sequence, expected amplicon sizes, and annealing temperature for the AcH 505 and P. croceum primers

From: Detection and quantification of a mycorrhization helper bacterium and a mycorrhizal fungus in plant-soil microcosms at different levels of complexity

Target

Amplicon size (bp)

Primer sequence (5′ → 3′)

Annealing temp. (°C)

AcH 505, intergenic region between gyrA/gyrB genes

107

AcH107-f (GGCAAGCAGAACGGTAAGCGG)

55

AcH107-r (TGGTCGGTGTCCATCGTGGT)

P. croceum, ITS

121

ITSP1-f (GGATTTGGAGCGTGCTGGCGT)

55

ITSP1-r (TTGTGAGCGGGCTTTTCGGACC)

P. croceum, intergenic region between two ORFs

127

Pilo127-f (GTCAGAGACGGACGCAGTTG)

62

  

Pilo127-r (CCAGTCAGCGGAGGAGAA)