Strain/Plasmid | Relevant characteristics | Source/ Reference |
---|---|---|
E. coli | Â | Â |
DH5α | Cloning strain, recA endA1 gyrA96 thi-1 hsdR17 supE44 relA1 ϕφ80 ΔlacZΔM15 | Gibco-BRL |
S. aureus | Â | Â |
NCTC8325-4 | MSSA strain | R. Novick |
BCBHV008 | NCTC8325-4Δspa::P spac -MCS-lacI lacI mc, Cmr | [23] |
8325-4ΔrecU | NCTC8325-4 recU mutant lacking initial 165 codons | This study |
8325-4recUspaL | NCTC8325-4 Δspa::P spac -recU-lacI | This study |
BCBRP001 | NCTC8325-4 ΔrecU Δspa::P spac -recU-lacI | This study |
8325-4recUi | NCTC8325-4 ΔrecU Δspa::P spac -recU-lacI lacI mc, Cmr | This study |
BCBHV017 | BCBHV008 strain expressing spoIIIE-yfp from the native chromosomal locus, Cmr | This study |
BCBRP002 | 8325-4recUi mutant strain expressing spoIIIE-yfp, Cmr | This study |
Plasmids | Â | Â |
pMAD | E. coli – S. aureus shuttle vector with the bgaB gene encoding a ß-galactosidase and thermosensitive origin of replication for Gram-positive bacteria, Ampr Eryr | [24] |
pBCB13 | pMAD derivative with P spac -lacI between up- and downstream regions of the spa gene, Ampr Eryr | [25] |
pMGPII | Plasmid encoding lacI gene; Cmr | [26] |
pMADrecUKO | pMAD derivative used for deletion of the first 165 codons of recU, Ampr Eryr | This study |
pBCB13recUspaL | pBCB13 derivative containing P spac -recU-lacI Ampr Eryr | This study |
pMUTINYFPKan | Integrative vector for C-termini YFP fusions; Ampr, Kanr | [27] |
pBCBHV007 | pMUTINYFPKan containing spoIIIE–yfp Ampr, Kanr | This study |
pMADspoIIIEyfp | pMAD derivative containing 3’ end spoIIIE –linker-yfp, Ampr Eryr | This study |
pBCBHV008 | pMAD derivative containing 3’ end spoIIIE–linker-yfp-3’ 64 bp and downstream region of spoIIIE, Ampr Eryr | This study |