Fluorescence amplified fragment length polymorphism compared to pulsed field gel electrophoresis for Listeria monocytogenes subtyping

Table 3 PFGE and fAFLP typing results from a panel of 97 L. monocytogenes isolates with index of discrimination (ID)

*L. monocytogenes*lineages	Serogroups¹or serotype²	Number of isolates	Number of PFGE³types	PFGE ID⁴	Number of fAFLP³types	fAFLP ID⁴
I	IVb	35	36	0.988	33	0.981
I	IIb	11	36	0.988	33	0.981
II	IIa	45	45	0.995	43	0.989
II	IIc	5	45	0.995	43	0.989
III	4a	1	1	n/a	1	n/a
Total:		97	82	0.996	76	0.993

¹ Serogrouping performed by multiplex PCR [4]: results are from both the European Reference Laboratory (EURL) for L. monocytogenes and the UK National Reference laboratory (UK-NRL) for Listeria.
² Based on sero-agglutination performed by EURL.
³ApaI/AscI-PFGE was performed by the EURL and HindIII/HhaI-fAFLP by UK-NRL.
⁴ Index of discrimination calculated according to the Simpson’s index of diversity (ID) [25]. n/a: not applicable.

ISSN: 1471-2180