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Table 1 PFGE and fAFLP discriminatory ability using Listeria monocytogenes isolates of duplicate strains, associated with outbreaks or with sporadic cases

From: Fluorescence amplified fragment length polymorphism compared to pulsed field gel electrophoresis for Listeria monocytogenes subtyping

Isolate

Test Study (TS) group number[17]

Responsible for sporadic (S) or outbreak (OB). Duplicate culture (D)

Origin of isolate

Country of origin

Molecular serogroup1

PFGE2ApaI/AscI type

fAFLP2HhaI/HindIII type

10CEB565LM

n/a

OB 1

Human

England

IVb

326/136

IV4.3

10CEB567LM

n/a

OB 1

Food

England

IVb

326/136

IV4.3

10CEB550LM

n/a

OB 2

Human

England

IVb

178/6

I.8

10CEB552LM

n/a

OB 2

Food

England

IVb

178/6

I.8

10CEB553LM

n/a

OB 3

Human

England

IIa

149/109

III.10

10CEB554LM

n/a

OB 3

Food

England

IIa

149/109

III.10

10CEB559LM

n/a

OB 4

Human

England

IVb

309/142

UD4.1

10CEB560LM

n/a

OB 4

Food

England

IVb

309/142

UD4.1

10CEB542LM = 10CEB543LM3

n/a

D

Human

England

IIc

70/377

VIIc.8

TS32

02

S

Food

USA

IVb

180/50

I.67

TS72

02

S

Food

USA

IVb

180/50

I.67

TS56 = TS773

03

S4 and D

Human

USA

IIa

120/191

VIIa.27

TS39

03

S

Food

USA

IIa

120/191

VIIa.27a

TS67

03

S4

Human

USA

IIa

120/191

VIIa.27a

TS17

05

S

Human

USA

IIb

93/140

IVb.21

TS61

05

S

Food

USA

IIb

93/140

IVb.21

TS31

15

OB 5

Human

France

IVb

24-Dec

V.21

TS69

15

OB 5

Human

France

IVb

24-Dec

V.21

TS21

16

OB 6

Food

Switzerland

IVb

19/15

V.3

TS55

16

OB 6

Human

Switzerland

IVb

19/15

V.3

TS02

22

S25

Human

England

IIc

70/25

VIIc.1

TS08

22

S25

Human

England

IIc

70/25

VIIc.1

  1. 1 Serogrouping performed by multiplex PCR [4]: results are from both the European Reference Laboratory (EURL) for L. monocytogenes and the UK National Reference laboratory (UK-NRL) for Listeria.
  2. 2 PFGE was performed by the EURL and fAFLP by UK-NRL.
  3. 3 Serogrouping and typing results were the same for each of the duplicate culture.
  4. 4 The 2 patients of TS group number 3 were 2 separate sporadic cases and not epidemiologically linked [18].
  5. 5 These 2 isolates are from the same patient who had 2 recurrent episodes of listeriosis [19].
  6. n/a: not applicable.