Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans

Table 4 Oligonucleotides used for the amplification of gene fragments with PCR

Primer	Sequence (5′-3′)	T_a(°C)	Protein encoded by the target gene	Product size (bp)	Reference
pufLF1	CTK TTC GAC TCC TGG GTS GG	56	Reaction center proteins L and M subunits	1500	[5]
pufMR1	CCA TSG TCC AGC GCC AGA A	56	Reaction center proteins L and M subunits	1500	[5]
pufLF2	CTY TTT GAY TTC TGG GTD GG	56	Reaction center proteins L and M subunits	1500	This study
pufMR2	CCA TSG TCC AGC GCC ARA A	56	Reaction center proteins L and M subunits	1500	This study
PR1	MGN TAY ATH GAY TGG YT	47	Proteorhodopsin opsin subunit	312	[26]
PR2	WWN MGN TAY GTN GAY TGG
PR3	GGR TAD ATN GCC CAN CC
soxB432F-2	GAY GGN GGN GAY MYB TGG	54	Sox enzyme complex B subunit	1000	This study
soxB1446B-2	CAT RTC WCC MCC YTG YTG	54	Sox enzyme complex B subunit	1000	This study
rpoB-F	AAY CAG TTC CGC GTN GGH YTN GT	52	RNA polymerase beta subunit	1000	This study
rpoB-R	AAG TTR TAR CCR TTC CAR GGC AT	52	RNA polymerase beta subunit	1000	This study

The IUPAC nucleotide code is used to indicate wobble positions in degenerate primer sequences. T_a indicates the annealing temperature used in the PCR reaction.

ISSN: 1471-2180