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Table 6 PCR primer sets used in this study

From: Genomic variations associated with attenuation in Mycobacterium avium subsp. paratuberculosisvaccine strains

Gene set

Sequence

vGI-1b: (internal gene primer sets)

 

MAP0101.F

GGTTACCGACTTGGTCCAGA

MAP0101.R

CCCGTCAGATCCATTACGAC

MAP0103.F2

GAGCGCCAACCTATCTCAAC

MAP0103.R

TGGTTTGGAGATCCCTGAAG

vGI-19 (internal gene primer set)

 

MAP3733c.F

TTCATGTTGTTCCTCACCCG

MAP3733c.R

CATCGCGAGCGTAGCCGCTG

vGI-20 (internal gene primer set)

 

MAP1723.F

GGAGTATGGAACCATCGCTG

MAP1723.R

TGATGTAACGGGCGTGCAAG

vGI-21 (specific for tandem duplicated region in IIUK2000/1)

 

MAP2705.SEQ2

CGATGAAGGCCTACTGGTC

MAP2733.R

TCAACTGGCTCCTCCTTTTG

vGI-22 (specific for tandem duplicated region in 316FNLD1978)

 

MAP1789.F

TTTGGCACTACAACGAGCTG

MAP1750.R

GATCGAGAAGAGGGGAGTCA

MAP2114c (single copy gene for control PCR)

 

MAP2114c.F

TGGAAGCTACCCAAATCACC

MAP2114c.R

GAGAAGTAGGCCGCGTAGTC

IS900

 

AV-1

ATGTGGTTGCTGTGTTGGATGG

AV-2

CCGCCGCAATCAACTCCAG

pre16SrRNA

 

pre16SrRNA.R

GCGCAGCGAGGTGAATTT

pre16SrRNA.F

TTTGGCCATACCTAGCACTCC

Tellurite (tehB)

 

MAP3730.F

GGTTTGACGACAGAGATGCG

MAP3730.R

GGCATGGTGTACGACAAGGA

MIRU3 (MAP3981-2)

 

MIRU3.F

ACATTCACCCTGTCCATTCC

MIRU3.R

CCTCCTTACGGAGCAGGAA

IS900(MAP0034)

 

MAP0033c.F

AGCTGCCGGGAGTTGATCT

MAP0035.R

TCACGGCTCACCGCACGCT

IS900(MAP0159c)

 

MAP0158.F

CCCGTGACAAGGCCGAAGA

MAP0160.R

CGTTTTGCACCTCGATGGCC

IS900(MAP0967)

 

MAP0966c-MAP0967.F

CGTGACGAACGACATGTGTT

MAP0967-MAP0968.R

GCTCGAGACATTTAGCCCAC

IS900(MAP1033)

 

MAP1032c.F

GTAGAACCCGACCAGCAGC

MAP1034c.R

GACCTGGCACCTGTATCCC

IS900(MAP1722)

 

MAP1720-MAP1721c.F

CTAAGGTGGCCAGCGTTTCT

MAP1723.R

GGTTGGAGACAACCTCGTTC

IS900(MAP1771c)

 

MAP1770c.F

ACAATTCGGCGATCGTCTCG

MAP1772.R

CGCGGACAGACACAGGTAGG

IS900(MAP1785)

 

MAP1784c.F

GTCGACCATCGCTCTTCCCT

MAP1786c.R

ATCGGTGTCGAGGACATTCC

IS900(MAP1793c)

 

MAP1792.F

CAATGGATGGTCGTCACCTG

MAP1794.R

CGGCCCGCTTGATCCATTTG

IS900(MAP2034c)

 

MAP2033-MAP2034c.F

CCGCAAGTAGTGCACTATGG

MAP2035.R

TATTCGGGGTTGTTCAGGGA

IS900(MAP2108)

 

MAP2105.F

CAGGCACGGAACACAGTTCG

MAP2109c.R

TGTTCGGCTACGGCATACTG

IS900(MAP2157)

 

MAP2156.F

CTGCAAACACAGCCCAATC

MAP2158.R

CAACTTCGGCAAGTTCACC

IS900(MAP2203c)

 

MAP2202c.F

TCCCGGTAGAAGATCATGTG

MAP2204c.R

GACAATCTGCCGTCGTATCA

IS900(MAP2444c)

 

MAP2443.F

AACCTTGACCCACACCTTCC

MAP2445.R

TGAGCTCGCCGGCGAAATA

IS900(MAP2577)

 

MAP2567c.F

CGTTCTGGGCATCCATCGACG

MAP2578.R

TCACGGCGGTCAGGTTACTTC

IS900(MAP3480)

 

MAP3479c.F

GTTGAACTTTCCGACCAACC

MAP3480-3481.R

GGTTAGCGGGAGAAAAGCTC

IS900(MAP4066)

 

MAP4065.F

TGGGCCTGAGGTCAGAACCA

MAP4067c.R

GAAGACCACCTCTACCTCAC

IS900(MAP4281)

 

MAP4280.F

GCTGACCGAGAAGGGCTAC

MAP4282.R

CGTAAGTGACTGGCTCATGG

  1. MAP gene annotation corresponds to GenBank AE016958.