Figure 3

Measurement of TGF-β2 mRNA and protein level. NCI-H292 cells with or without treatment of miR-141 inhibitor, were infected with influenza A virus subtypes: H1N1/2002 or H5N1/2004 viruses at m.o.i. = 1, respectively for 24 hours. qRT-PCR were used to quantitify the TGF-β2 mRNA levels and fold-changes were calculated by ΔΔCT method as compared with non-infection cell control (mock) and using endogeneous actin mRNA level for normalization. TGF-β2 protein level was measured by enzyme-linked immunosorbent assay as compared with mock. Each point on the graph respresents the mean fold-changes. The experimental mean fold-changes of mRNA and protein levels were compared to that of mock controls ± SD (p* < 0.05), (p^#< 0.05), respectively.