Table 4 Primers for construction of the arp mutagenic cassette and verification of allelic exchange
From: Influence of arthritis-related protein (BBF01) on infectivity of Borrelia burgdorferi B31
Primer | Sequence (5′ > 3′) | Application |
|---|---|---|
ARP01 | GCCTTTCGTTAAGGTTTTGTTT | amplify arp upstream homology |
ARP02 | GGAAATCTTCCTTGAAGCTCGGGTACAA | SOEing arp upstream homology |
| Â | GTTGTTCCTCCTAAATTAAATAAAAATAA | to aadA cassette |
ARP03 | TACCCGAGCTTCAAGGAAG | amplify aadA cassette |
ARP04 | GGTATATGTAATTTCGACTTTAAGTTAAAAAT | SOEing arp downstream |
| Â | CCGATTGTTTCATTTGCCGACTACCTTGGT | homology to aadA cassett |
ARP05 | GAACAATCGGATTTTTTAACTTAAAGTCG | amplify arp dowsteam homology |
ARP06 | ACCCCAGTAACTCAATTTCTAATTG | amplify arp dowsteam homology |
ARP07 | TTTCTTGATTAGGGTAAAAAATTCT | check integration at 5′ end |
ARP08 | GTCTTGTATTGTTGAACAAAACACTT | check integration at 3′ end |
ARP09 | GTTTCCATATGAGGGAAGCG | check integration within aadA |
ARP10 | CCAAGCGATCTTCTTCTTGTC | check integration within aadA |