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Figure 6 | BMC Microbiology

Figure 6

From: Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes

Figure 6

Transcriptional expression patterns of the three Bdellovibrio chaperonin genes across the wild type predatory cycle. RT-PCR with transcript-specific primers was performed on total RNA prepared from identical volumes of B. bacteriovorus HD100 predator with E. coli S17-1 prey infection culture as the predatory infection proceeds across a time course. L- NEB 100 bp ladder, AP- attack-phase 15–15 minutes predation, 30–30 minutes predation, 45–45 minutes predation 1-4 h: 1,2,3,4 hours predation respectively. Controls of no template, no reverse transcriptase, E. coli S17-1 only RNA as template and bacteriovorus HD100 genomic DNA were carried out. Primers designed to bd0097 groES1 and bd0099 groEL give a product in every sample, thus act as a positive control for the RNA, validating the lack of expression of bd3349 groES2 in the earlier part of the infectious cycle.

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