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Figure 3 | BMC Microbiology

Figure 3

From: Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes

Figure 3

RT-PCR showing relative levels of transcription of chaperonin and flagellin genes in total RNA from attack phase Bdellovibrio , under normal and heat-shocked conditions. RT-PCR with transcript specific primers was carried out on matched concentrations of RNA (matched by Nanodrop spectrophotometer readings) from wild-type and mutant attack-phase Bdellovibrio including samples subjected to heat shock (42°C for 10 minutes). Total RNA samples from :-WT- wild-type HD100 attack phase, N- non-heat shocked 29°C, HS- heat shocked at 42°C for 10 minutes, 0881- ΔBd0881 attack phase, 0743- ΔBd0743 attack phase, Lane 7- no template negative control, Lane 8- HD100 genomic DNA positive control. “No reverse transcriptase” controls were performed for each template and were negative for DNA contamination (data not shown). The abundant transcript produced using primers designed to anneal to the fliC1 gene acts as a positive control by showing that there was ample total RNA in all samples.

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