Figure 3
From: Anti-staphylococcal activities of lysostaphin and LytM catalytic domain
Pulldown assay with S. aureus cell walls treated in various ways. Pulldown of (A) lysostaphin, (B) LytM185-316 and (C) LytM26-316 with S. aureus cell walls treated in various ways. (1) Input, (2) sonicated crude cell walls, (3) washed crude cell walls, (4) SDS-treated cell walls, (5) TCA-treated cell walls, (6) trypsinised cell walls, (7) purified peptidoglycans (8) commercially available peptidoglycans. The protein that was input (lane 1) or pulled down (lanes 2–8) was visualized by Western blotting with the anti-LytM antibody.