Unbalance of intestinal microbiota in atopic children

BMC Microbiology

Table 2 Primer sets used for the 16S rRNA gene quantification of A. muciniphila , F. prausnitzii , Enterobacteriaceae , Clostridium cluster IV, Bifidobacterium and Lactobacillus group by qPCR. Amplicon size, annealing and fluorescence acquisition temperature are also reported

Target microorganism	Primer set	Sequence (5' to 3')	Product size (bp)	Annealing temp (°C)	Fluorescence acquisition temp (°C)	Reference
Akkermansia muciniphila	AM1	CAGCACGTGAAGGTGGGGAC	349	63	88	[31]
	AM2	CCTTGCGGTTGGCTTCAGAT
Faecalibacterium prausnitzii	Fprau223F	GATGGCCTCGCGTCCGATTAG	199	67	85	[32]
	Fprau420R	CCGAAGACCTTCTTCCTCC
Enterobacteriaceae	Eco1457F	CATTGACGTTACCCGCAGAAGAAG	195	63	87	[32]
	Eco1652R	CTCTACGAGACTCAAGCTTGC
Clostridium Cl_IV	S-*-Clos-0561-a-S-17	TTACTGGGTGTAAAGGG	588	60	85	[33]
	S-*-Clept-1129.a-A-17	TAGAGTGCTCTTGCGTA
Bifidobacterium	bif-164	GGGTGGTAATGCCGGATG	523	60	90	[34]
	bif-662	CCACCGTTACACCGGGAA
Lactobacillus group	Lac1	AGCAGTAGGGAATCTTCCA	327	61	85	[35]
	Lac2	ATTYCACCGCTACACATG

ISSN: 1471-2180