Characterization of LysB4, an endolysin from the Bacillus cereus-infecting bacteriophage B4

BMC Microbiology

Table 2 The antimicrobial spectrum of LysB4

Organisms		Relative lytic activity (%)
Gram-negative bacteria	Escherichia coli MG1655	++
	Escherichia coli O157:H7 ATCC 43894	++
	Escherichia coli O157:H7 ATCC 43890	++
	Escherichia coli O157:NM 3204-92	++
	Pseudomonas aeruginosa ATCC 27853	++
	Cronobacter sakazakii ATCC 29544	++
	Shigella flexineri 2a strain 2457 T	+
	Shigella boydii IB 2474	++
	Salmonella Typhimurium LT2	+
	Salmonella Enteritidis ATCC 13078	+
Gram-positive bacteria	Listeria monocytogenes ATCC 19114	++
	Bacillus cereus ATCC 40133	+++
	Bacillus cereus ATCC 27348	+++
	Bacillus subtilis 168	+++
	Enterococcus faecalis ATCC 29212	-
	Staphylococcus aureus ATCC 29213	-
	Lactococcus lactis subsp. Lactis ATCC 11454	-
	Streptococcus thermophilus ATCC 19258	-

Gram-negative bacteria were treated with EDTA. Relative lytic activity was obtained by comparing the lytic activity of each test to it toward B. cereus ATCC10876; 1-40% +, 41-70% ++, 71-100% +++, 0% -

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