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Table 2 Bacterial strains and plasmidsa

From: The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri

Strains and plasmids

Characteristics

Reference or source

Strains

  

E. coliDH5α

F- recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ (argE-lacZYA)169 φ80 lazA Δ M15

[29]

HB101

F- supE44, hsdS20(rB- mB-), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5, mtl-1, leuB6, thi

[30]

X. citri subsp. citri

  

306

Syn. X. axonopodis pv. citri strain 306; wild type, Rfr

[31]

223G4 (gpsX-)

gpsX (XAC3110):: EZ-Tn5 derivative of strain 306, Kmr, Rfr

[24]

223G4V (gpsX-)

223G4 (gpsX-) containing pUFR053, Cmr, Gmr, Kmr, Rfr

This study

C223G4 (gpsX+)

223G4 (gpsX-) containing pJU3110, Cmr, Gmr, Kmr, Rfr

This study

Plasmids

  

pRK2013

ColE1 Kmr Tra+, conjugation helper plasmid

[32]

pUFR053

IncW Mob+mob(P) lacZ+ Par+, Cmr, Gmr, shuttle vector

[33]

pJU3110

2,299-bp KpnI- HindIII fragment containing wild-type gpsX cloned in pUFR053; Cmr, Gmr

This study

  1. aApr, Cmr, Gmr, Kmr, and Rfr indicate resistance to ampicillin, chloromycetin, gentamicin, kanamycin and rifamycin, respectively.