Strains and plasmids | Characteristics | Reference or source |
---|---|---|
Strains | Â | Â |
E. coliDH5α | F- recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ (argE-lacZYA)169 φ80 lazA Δ M15 | [29] |
HB101 | F- supE44, hsdS20(rB- mB-), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5, mtl-1, leuB6, thi | [30] |
X. citri subsp. citri | Â | Â |
306 | Syn. X. axonopodis pv. citri strain 306; wild type, Rfr | [31] |
223G4 (gpsX-) | gpsX (XAC3110):: EZ-Tn5 derivative of strain 306, Kmr, Rfr | [24] |
223G4V (gpsX-) | 223G4 (gpsX-) containing pUFR053, Cmr, Gmr, Kmr, Rfr | This study |
C223G4 (gpsX+) | 223G4 (gpsX-) containing pJU3110, Cmr, Gmr, Kmr, Rfr | This study |
Plasmids | Â | Â |
pRK2013 | ColE1 Kmr Tra+, conjugation helper plasmid | [32] |
pUFR053 | IncW Mob+mob(P) lacZ+ Par+, Cmr, Gmr, shuttle vector | [33] |
pJU3110 | 2,299-bp KpnI- HindIII fragment containing wild-type gpsX cloned in pUFR053; Cmr, Gmr | This study |