Figure 4From: The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citriGpsX is important for host virulence of X. citri subsp. citri. (A) Suspensions of each strain [approximately 105 and 108 cfu/ml, respectively] were inoculated into the intercellular spaces of fully expanded, immature grapefruit (C. paradise cv. Duncan) leaves by pressure infiltration with a needleless syringe. A representative leaf from four replicates was photographed at 7 and 14 dpi, respectively. W: wild-type strain 306; M: gpsX mutant 223 G4 (gpsX-); MV: gpsX mutant 223G4V (gpsX-) with empty vector pUFR053; CM: complemented gpsX mutant C223G4 (gpsX+). (B) Bacterial cell suspensions (approximately 108 cfu/ml) of wild-type strain 306 and its derivatives were inoculated onto fully expanded, immature grapefruit by spray. Images are representative of five independent replicates at 21 dpi.Back to article page