Table 3 Primers used for yitR amplification, creation of KIM6+Δ yitA-yipB, and creation of YitA and YipA-β-lactamase fusion proteins
Primer Designation | Sequencea |
|---|---|
YitR Forward Primer | 5′-AGTTGAGCTCGTCTGCATTGATTATTTGACC-3′ |
YitR Reverse Primer | 5′-AGTTTCTAGAGATCGTTGCGTAGCTGTGTTGC-3′ |
YitA-YipB KO Primer Forward | 5′-TGGCATCAATAAACTGGCCTTTTCTGTTGCAC |
CAAAAATATGTGTAGGCTGGAGCTGCT-3′ | |
YitA-YipB KO Primer Reverse | 5′-TTCCCTATTCAAAATAGGGAAGGTGTTTAAAA |
TTAATAAACATATGAATATCCTCCTTA-3′ | |
YitA C-terminus Forward | 5′-ATCTCTAGACCCCAGAACACCCCATGTAT-3′ |
YitA C-terminus Reverse | 5′-GATCTCGAGAAGATTAACTCTTAGCTTGT-3′ |
YitA Downstream Forward | 5′-ATCCTCGAGGTCGACAAAAACGTTATTACATCCAA-3′ |
YitA Downstream Reverse | 5′-GATGCATGCCTGATTTGACTGATTTTTCC-3′ |
YipA C-terminus Forward | 5′-ATCTCTAGAAGAGACTTAAAGCATATGGT-3′ |
YipA C-terminus Reverse | 5′-GATCTCGAGTCGATTCTGTTTGCTATATA-3′ |
YipA Downstream Forward | 5′-ATCCTCGAGGTCGACTTAAGAATATTAAGGAGCCA-3′ |
YipA Downstream Reverse | 5′-GATGCATGCTGGCCGTTCAGGTTGCAGTT-3′ |
Mature β-lactamase Forward | 5′-ATCCTCGAGCACCCAGAAACGCTGGTGA-3′ |
Mature β-lactamase Reverse | 5′-GATGTCGACTTACCAATGCTTAATCAGTGA-3′ |