Figure 4

YitA and YipA proteins persist in Y. pestis for at least 9 hours after transfer to 37 °C . Y. pestis KIM6+ (pCR-XL-TOPO::yitR) (lanes 5, 8, 11, 14, 18, 21, 24, and 27) and KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) (lanes 3, 6, 9, 12, 15, 19, 22, 25, and 28) were grown overnight at 22°C and subsequently transferred to 37°C for the indicated amount of time prior to sample collection. A matched KIM6+ (pCR-XL-TOPO::yitR) was maintained at 22°C as a positive reference control (lanes 2, 4, 7, 10, 13, 17, 20, 23, and 26). T0 = initial time point, T(x) = x hours at 37°C. Panels show Western blots probed with anti-YitA, anti-YipA, or anti-Ail (sample loading control) antiserum.