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Figure 3 | BMC Microbiology

Figure 3

From: Characterization of the Bat proteins in the oxidative stress response of Leptospira biflexa

Figure 3

Quantitative RT-PCR analysis of the bat locus and downstream genes. Gene targets are shown below the corresponding section of the bar-graph using specific primer-probe sets for each gene (Table 1). Transcript from each gene was normalized to 104 copies of flaB transcript from the respective strain. –X–, indicates deletion of the corresponding gene indicated above. Values represent the mean of triplicate reactions ± the standard error. Unpaired T test with Welch’s correction was used to determine significant differences between two groups (e.g. batB transcript levels between WT and ΔbatA mutant strains). For statistical analysis of more than 2 groups (such as comparisons of gene transcripts between WT, ΔbatA mutant and ΔbatABD mutant strains), one-way analysis of variance (ANOVA) with the Bonferroni’s post test was applied. P values < 0.0001 are denoted by ***.

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