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Figure 2 | BMC Microbiology

Figure 2

From: Characterization of the Bat proteins in the oxidative stress response of Leptospira biflexa

Figure 2

Gene organization in wild-type and mutant strains of L. biflexa . (A) Genetic organization of bat genes and flanking genes on chromosome II of L. biflexa (not drawn to scale). The corresponding deleted regions in mutant strains are depicted with the respective bat genes replaced by the kanamycin-resistance cassette [13]. (B) Southern blot analysis of L. biflexa strains confirms the absence of the respective bat genes in mutant strains. Genomic DNA for the Southern blot was double-digested with restriction endonucleases NdeI and PstI. Three independently isolated transformants from each mutant were compared to wild-type and hybridized with either a labeled batA fragment or with a labeled fragment spanning batB to batD. The weak signal observed at ~3 kb in the batA mutant strains hybridized with the batA probe is likely due to cross-hybridization with batB. +, purified plasmid DNA from E. coli with a cloned region of L. biflexa DNA containing batABD.

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