Skip to main content

Table 1 B. pseudomallei P2-like prophage distribution screen

From: φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. malleistrain infectivity

   P2-like prophage PCR probe results
   Multiplex    
B. pseudomallei Candidate P2-like prophage group a P2-like 1 P2-like 2 P2-like group A φX216 scrnA φX216 scrnB GI2
Strains with high φX216 plaquing efficiencyb66 .7% (22/33) P2-like prophage candidate positive strains  
2668a φ52237-like + + + + + +
E0237 c φ52237-like + + + + + +
E0394 φ52237-like + + + + + +
1026b d φ52237-like + + + + + +
708a φ52237-like e + + + + - +
2618a P2L-A + + + - - +
2661a P2L-A + + + - - +
2692a P2L-A + + + - - +
2717a P2L-A + + + - - +
E0021 P2L-A + + + - - +
E0235 P2L-A + + + - - +
E0279 P2L-A + + + - - +
E0345 P2L-A + + + - - +
E0384 P2L-A + + + - - +
E0386 P2L-A + + + - - +
K96243 f P2L-A + + + - - +
S13 g P2L-A + + + - - +
2698a P2L + + - - - -
2704a P2L h + + - - + -
E0342 P2L + + - - - -
E0366 P2L + + - - - -
E0377 P2L + + - - - -
2613a   - - - - - ND i
2667a   - - - - - ND
2673a   - - - - - ND
2682a   - - - - - ND
2769a   - - - - - ND
E0016   - - - - - ND
E0034   - - - - - ND
E0241   - - - - - ND
E0356   - - - - - ND
E0411   - - - - - ND
MSHR305   - - - - - ND
Strains with low φX216 plaquing efficiency j17. 4% (4/23), P2-like prophage candidate positive strains  
2625a φ52237-like + + + + + +
2670a P2L-A + + + - - +
E0037 P2L-A + + + - - +
E0380 P2L-A + + + - - +
2637a   - - - - - ND
2650a   - - - - - ND
2660a   - - - - - ND
2685a   - - - - - ND
2708a   - - - - - ND
2719a   - - - - - ND
2764b   - - - - - ND
E0024   - - - - - ND
E0031   - - - - - ND
E0181   - - - - - ND
E0371   - - - - - ND
E0372   - - - - - ND
E0378   - - - - - ND
E0383   - - - - - ND
E0393   - - - - - ND
1710a   - - - - - ND
1710b k   - - - - - -
1106b   - - - - - ND
406e   - - - - - ND
Non-φX216 plaquing strains 25. 0% (4/16), P2-like prophage candidate positive strains  
2671a P2L-A + + + - - +
2674a P2L-A + + + - - +
2677a P2L-A + + + - - +
Pasteur 6068 P2L-A + + + - - +
2614a   - - - - - ND
2617a   - - - - - ND
2640a   - - - - - ND
2665a   - - - - - ND
2689b   - - - - - ND
2694a   - - - - - ND
E0008   - - - - - ND
E0183   - - - - - ND
E0350   - - - - - ND
E0396   - - - - - ND
1106a   - - - - - ND
MSHR668   - - - - - ND
  1. a φ52237-like assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A, and individual PCR probes φX216 scrnA, φX216 scrnB and GI2. P2L-A assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A and individual PCR probe GI2. P2L assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2.
  2. bConfluent lysis when spot tested with ~106 pfu φX216.
  3. cφX216 source strain.
  4. d1026b φ52237-like prophage is split into two segments and likely non-functional [15].
  5. eP2-like prophage group cannot be determined based on PCR results. May be P2L-A or φ52237-like.
  6. fφK96243 prophage (group P2-A) located at GI2 [9].
  7. gEncodes the predicted prophage PI-S13-1 (group P2-A) [88].
  8. hP2-like prophage group cannot be assigned based on PCR results. May be P2L or φ52237-like.
  9. IND, GI2 probe results not determined.
  10. jNon-confluent lysis / individual plaques when spot tested with ~ 106 pfu φX216.
  11. kThe strain 1710b genome does not contain a P2-like prophage or prophage insertion at GI2.