FK506 biosynthesis is regulated by two positive regulatory elements in Streptomyces tsukubaensis

BMC Microbiology

Table 1 Strains and plasmids used in this study

Strain	Plasmid	Promoter	Gene	# if isolates	Description
WT				11	Control
WT pSET152	pSET152			22	Control
WT allN	pDG1	P_ermE*	allN	28	Over-expression
WT allN+mgl	pDG2	P_ermE*	allN+mgl	25	Over-expression
WT fkbR	pDG3	P_ermE*	fkbR	24	Over-expression
WT fkbN	pDG4	P_ermE*	fkbN	29	Over-expression
ΔallN	pDG5		ΔallN	7	Gene inactivation
ΔallNΔfkbN	pDG5, 8		ΔallN ΔfkbN	2	Gene inactivation
ΔfkbN	pDG8		ΔfkbN	10	Gene inactivation
ΔfkbR	pDG6		ΔfkbR	6	Gene inactivation
ΔfkbRΔfkbN	pDG6, 8		ΔfkbR ΔfkbN	2	Gene inactivation
ΔfkbR	pDG3	P_ermE*	fkbR	18	Complementation
ΔfkbN	pDG4	P_ermE*	fkbN	33	Complementation
ΔfkbRΔfkbN	pDG4	P_ermE*	fkbN	9	Complementation
WT	pSET152		rppA	12	Negative control
ΔfkbR	pMB1	P_ermE*	rppA	9	Positive control
	pMB2	P_allA	rppA	15	Promoter activity
	pMB3	P_fkbR	rppA	15	Promoter activity
ΔfkbN	pMB4	P_fkbN	rppA	12	Promoter activity
ΔfkbN	pMB5	P_fkbB	rppA	15	Promoter activity
	pMB6	P_fkbG	rppA	15	Promoter activity

ISSN: 1471-2180