Table 1 Primers designed and used in this work
Nº | Primer | Sequence 5’ to 3’ | Target |
|---|---|---|---|
1 | H-out.F | CTCGATGAGCTGATGCTTTG | Hygromycin B resistance cassette |
2 | H-out.R | TCCATCACAGTTTGCCAGTG | Hygromycin B resistance cassette |
3 | Zeo.F | TGAACAGGGTCACGTCGT | Zeocin resistance cassette |
4 | Zeo.R | CGCTGATGAACAGGGTCAC | Zeocin resistance cassette |
5 | CYP61up2.F | CTGGAGCCGAATTCATTGAT | CYP61 gene |
6 | CYP61dw2.R | AGGAGGCAGAGTGGTTGAGA | CYP61 gene |
7 | CYP61b.F | GTCGGAGGAAGAGCAGTTTG | CYP61 gene |
8 | CYP61.F | CTGAGCCCTGTCTTGTTGCC | CYP61 gene |
9 | CYP61.R | ATTGTACACCTTTGTTCCAGGC | CYP61 gene |
RT-qPCR (The pairs of primers used had efficiency greater than 95%, as determined by standard curves with a correlation coefficient of R2 ≥ 0.996): | |||
10 | mactF-RT | CCGCCCTCGTGATTGATAAC | ACT gene |
11 | mactR-RT | TCACCAACGTAGGAGTCCTT | ACT gene |
12 | hmgR.F-RT | GGCCGATCGCTATACATCCGTTT | HMGR gene |
13 | hmgR.R-RT | ATCCAGTTGATGGCAGAAGGCT | HMGR gene |