Figure 6

RP-HPLC sterols analysis from UCD 67–385 and 385-cyp61^hph/cyp61^zeostrains. Chromatograms (at 280 nm) correspond to sterols extracted from strains as described in the Materials and Methods section. Beside each peak (peaks Nº 1 to 3), the corresponding spectra were included. Sterols were analyzed from UCD 67–385 wild-type (A), UCD 67–385 wild-type co-injected with standard ergosterol (B), 385-cyp61^hph/cyp61^zeo mutant (C) and 385-cyp61^hph/cyp61^zeo co-injected with standard ergosterol. Ergosterol corresponds to peak Nº 1.