Advancement of the 10-species subgingival Zurich Biofilm model by examining different nutritional conditions and defining the structure of the in vitrobiofilms

Table 2 Sequences, labels and formamide concentrations for FISH Probes

Organism	Name	Type	Labels	FA¹	WB²	Sequence (5’ → 3’)	References
A. oris	L-Act476-2	LNA³	Cy3, FAM, 6-Rox	40%	46 mM	ATCCAGCTACCGTCAACC	[11]
C. rectus	CAMP665	DNA	Cy3, Cy5	30%	112 mM	CATCTGCCTCTCCCTYAC	[11]
F. nucleatum	FUS664		Cy3, Cy5, FAM, FITC	40%	46 mM	CTTGTAGTTCCGCYTACCTC	[32]
	Fnuc133c	DNA	Cy3, Cy5	40%	46 mM	GTTGTCCCTANCTGTGAGGC	[11]
P. intermedia	L-Pint649-2	LNA	Cy3, FAM,6-Rox	40%	46 mM	CGTTGCGTGCACTCAAGTC	[11]
P. gingivalis	L-Pgin1006-2	LNA³	Cy3, Cy5, FAM	30%	112 mM	GTTTTCACCATCMGTCATC	[11]
Streptococci	STR405	DNA	Cy3, Cy5	20%	215 mM	TAGCCGTCCCTTTCTGGT	[34]
T. denticola	TrepG1_679	DNA	Cy3, Cy5, FAM	40%	46 mM	GATTCCACCCCTACACTT	[13]
T. forsythia	Tfor997	DNA	Cy3, Cy5, FAM	40%	46 mM	TCACTCTCCGTCGTCTAC	[35]
V. dispar	VEI217	DNA	Cy3, Cy5, FAM, FITC, 6-Rox	40%	46 mM	AATCCCCTCCTTCAGTGA	[32]

¹ Formamide concentration in the hybridisation buffer.
² Concentration of NaCl used in the washing buffer.
³ Probes containing locked nucleic acid substitutes (LNA). The ribose ring of LNA is constrained by a methylene linkage between the 2’ oxygen and the 4’ carbon.

ISSN: 1471-2180