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Table 2 Primer sequences

From: Expression of Shigella flexneri gluQ-rs gene is linked to dksAand controlled by a transcriptional terminator

Name

Sequence 5′- 3′a

Reference and characteristics

opeF

TAAGGAGAAGCAACATGCAAGA

This work. RT-PCR of dksA operon from nucleotide +40 to +1477b

opeR

ATAGCTCAGCATGACGCATTT

dksAF

ATGCAAGAAGGGCAAAACCG

This work. RT-PCR of dksA gene from nucleotide +54 to +488

dksAR

GCGAATTTCAGCCAGCGTTT

interF

AGTGGAAGACGAAGATTTCG

This work, RT-PCR of intergenic region from nucleotide +368 to +863

interR

TCCTTGTTCATGTAACCAGG

gQRSF

TTCAAAGAGATGACAGACACACAG

This work, RT-PCR of gluQ-rs gene from nucleotide +567 to +1074

gQRSR

CACGGCGATGAATGATAAAATC

rrsHF

CCTACGGGAGGCAGCAG

[40] RT-PCR of ribosomal RNA 16S

rrsHR

CCCCCGTCAATTCCTTTGAGTTT

pcnBR

GATGGAGCCGAAAATGTTGT

Reverse of pcnB gene from nucleotide +1993

PdksAF

GGATCCAAGCGAAGTAAAATACGG

BamHI site, from nucleotide −506

PdksARST

AAGCTTGTGATGGAACGGCTGTAAT

HindIII site, to nucleotide +527

PdksARCT

AAGCTTCTGTGTGTCTGTCATCTCTTTG

HindIII site, to nucleotide +590

PgluQF

GGATCCAAGAAGGGCAAAACCGTA

BamHI site, from nucleotide +58

TERGQ2

CCTTATTTTTTGTTCAAAGAGATGACAGACACACAGA

Recognition from nucleotide +555

TERMGQ3

ATAAGGCGGGAGCATAACGGAGGAGTGGTAAAC

Recognition from nucleotide +560, underline sequence are nucleotides changed

M13R

GCGGATAACAATTTCACACAGG

Recognition site in pTZ57R/T

ATGGQRSF

GGATCCGTAATTACAGCCGTTCCATC

BamHI site, from nucleotide +507. Underline nucleotides correspond to the stop codon of dksA

ATGGQRSR

CTCGAGGCATGACGCATTTGAGAATG

XhoI site, to nucleotide +1469

virFF

AGCTCAGGCAATGAAACTTTGAC

[41]

virFR

TGGGCTTGATATTCCGATAAGTC

  1. aNucleotides in bold are indicated restriction site.
  2. bFragments cloned are indicated based on the transcription start of dksA identified by [25].