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Table 1 Real-time PCR validation of RNA microarray results

From: Identification of the Streptococcus mutans LytST two-component regulon reveals its contribution to oxidative stress tolerance

 

Microarray

Real-time pcr

 

lytS mutant

lytS mutant

lrgAB mutant

 

(SMU.1985)

comYA (comYB)

22.9927

6.8449

0.8163

 

SMU.1967

ssbA

5.5803

4.1076

0.8791

 

(SMU.1515)

vicR (vicX)

2.6764

1.7647

1.0267

 

SMU.924

tpx

2.4148

3.6168

1.058

 

SMU.1739

fabF

2.2443

2.0333

1.084

 

SMU.1666

livG

2.1183

3.4331

1.009

 

SMU.80

hrcA

0.4953

0.6107

1.0204

 

SMU.1424

pdhD

0.4769

0.4031

1.2004

 

SMU.580

xseA

0.29849

0.5409

1.1398

 

SMU.1600

celB

0.2186

0.2825

1.2979

 

SMU.113

pfk

0.1597

0.176

1.3578

 

SMU.82

dnaK

0.1523

0.2652

0.9907

 

SMU.1344

fabD

0.0223

0.012

1.0637

 

SMU.1341

grs

0.0008

0.0121

1.1027

 
  1. Results are expressed in fold-change (mutant/wild-type).